000 05940cam a2200781Ia 4500
001 ocn162129261
003 OCoLC
005 20140426153707.0
006 m o d
007 cr cn|
008 070802s1995 caua ob 001 0 eng d
040 _aOPELS
019 _a182537805
020 _a9780120443093
020 _a0120443090
020 _a9780080536408 (electronic bk.)
020 _a0080536409 (electronic bk.)
029 1 _aNZ1
029 1 _aAU@
029 1 _aDEBBG
029 1 _aGBVCP
029 1 _aNLGGC
035 _a(OCoLC)162129261
037 _a88490:88490
_bElsevier Science & Technology
050 4 _aQR392
_b.V57 1995
060 0 _aQW 160
060 0 _aW1
070 _aQH430.M48
072 0 _aX300
072 7 _aSCI
082 0 4 _a576.6482
049 _aNTRA
245 0 0 _aViral gene techniques
_h[electronic resource] /
_cedited by Kenneth W. Adolph.
260 _aSan Diego :
_bAcademic Press,
300 _a1 online resource (xvii, 416 p.) :
490 1 _aMethods in molecular genetics,
_x1067-2389 ;
_vv. 7
520 _aViral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed. * Methods presented for ease of use and reasdy adaptation to new systems * Detailed experimental protocols included for: * Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus) * DNA viruses - virus/host interactions, viral chromosomes, transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus) * Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers * RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus).
505 0 _aViral Vectors: Adeno-Associated Virus: Integrating Vectors for Human Gene Therapy. Techniques for Human Adenovirus Vector Construction and Characterization. Construction and Isolation of Recombinant Adenoviruses with Gene Replacements. Construction and Use of Recombinant Vaccinia Virus Vectors. Epstein-Barr Viral Plasmid Vectors and Their Amplifiable Derivatives. DNA Viruses: Methods to Study Epstein-Barr Virus and P53 Status in Human Cells. The SV40 Minichromosome. Site-Specific Integration of Multigenic Shuttle Plasmids Into the Herpes Simplex Virus Type 1 (HSV. 1). Genome Using a Cell-Free Cre-Lax Recombination System. Strategies Transcription Regulation of Neurotropic Viruses in CNS: Lesson from JC Virus. Analysis of the Nuclear DNA-Binding Activity in Cells Overexpressing Hepatitus B Viral X and 3'-Truncated S Transactivators. Investigations on Virus-Host Interations: An Abortive Systems. Human Immunodeficiency Virus/Retroviruses: Quantitation of Virus Stocks Produced from Cloned Human Immunodeficiency Virus DNA. Quantitation of HIV-1 RNA in Plasma or Serum Samples. Detection of Cell Fusion Mediated By the Envelopes of Human Retroviruses By Transactivation of a Reporter Gene. Assembly of Recombinant Retroviral Gag Precursors Into Pseudovirions in the Baculovirus-Insect Cell Expression System. Retrovirus Reverse Transcription and Integration. In Vitro Binding and Transcription Assays Using the Human T-Cell Leukemia Type I Tax Protein. PCR-Based Techniques for Utilizing Retroviruses as Cell Lineage Markers. RNA Viruses: New Methods to Study Poliovirus Assembly and Encapsidation of Genomic RNA. Molecular Studies of Hepatitis Delta Virus Rnas. Systems to Express Recombinant RNA Molecules By the Influenza a Virus Polymerase in Vivo. Analysis of Membrane -Association in Vivo and Targeting of Viral Proteins in Polarized Epithelial Cells. Cloning of Viral Dsrna Genomes By Single Primer Amplification. Using the RNA-Capture Assay to Assess the RNA-Binding Activity of Viral Proteins. Protein RNA-Binding Activity Measured By Northwestern Blot Analysis: The Interferon-Inducible, RNA-Dependent Protein Kinase PKR. Index.
504 _aIncludes bibliographical references and index.
588 _aDescription based on print version record.
650 0 _aViral genetics.
650 7 _aSCIENCE
_xLife Sciences
650 0 7 _aViren.
650 0 7 _aGentechnologie.
650 0 7 _aAufsatzsammlung.
650 0 7 _aMolekulargenetik.
650 4 _aGenetics
650 4 _aAdenoviruses.
650 4 _aRetroviruses.
650 4 _aRNA viruses.
650 2 _aGenes, Viral
650 2 _aGenetic Techniques.
650 2 _aAdenoviridae
650 2 _aDNA Viruses
650 2 _aRNA Viruses
650 2 _aRetroviridae
653 0 _aViruses
655 4 _aElectronic books.
700 1 _aAdolph, Kenneth W.,
776 0 8 _iPrint version:
_tViral gene techniques.
_dSan Diego : Academic Press, c1995
830 0 _aMethods in molecular genetics ;
_vv. 7.
856 4 0 _3ScienceDirect
938 _aYBP Library Services
938 _aebrary
938 _aEBSCOhost
942 _cEB
994 _a92
999 _c170