Evaluation of Enzyme mixture for Enzymatic Hydrolysis and Ethanol Production from wheat straw

By: Sahoo, Shibashish SujitContributor(s): Singh, R K [Supervisor] | Department of Chemical EngineeringMaterial type: TextTextLanguage: English Publisher: 2014Description: 72 pSubject(s): Chemical EngineeringDissertation note: Thesis M.Tech (R) National Institute of Technology, Rourkela Summary: Cellulases and related hydrolytic enzymes represent a key cost factor for biochemical conversion of cellulosic biomass feedstocks to sugars for biofuels and chemical production. The performance of benchmark cellulose preparations produced by enzymes to convert pretreated wheat straw cellulose to glucose and fermentation to produce ethanol was evaluated under common experimental conditons and is reported here in a non-attributed manner. In the study, inspections were carried out with three commercial cellulose complexes produced by X, Y and Z enzymes. These set of enzymes were tested on pretreated wheat straw for enzymatic hydrolysis and ethanol production with simultaneous saccharification and fermention. For enzymatic hydrolysis, pretreated wheat straw (loaded at10% w/w ) was hydrolyzed with all three complexes at varied enzyme concentration ( 1 mg -30 mg protein/g of biomass, 50◦C, pH 5) separately in 250 ml roller bottles. Same set of enzymes were also used along with thermotolerantyeast (Saccharomycescerevisiae2034 ; 38◦C, pH 5) to produce ethanol using simultaneous saccharification and fermention process in 250 ml shak flask. In enzymatic hydrolysis, enzyme from X was the best performer ( high glucose yield at lower enzyme (protein) loadings) with up to95% glucose yield at roughly 10-15 mg protein/g cellulose , when compared with the enzymes from Y and Z. In simultaneous saccharification and fermention, Ethanol yield with enzyme from X was upto 80% at 20 mg protein/g cellulose which was comparably high when compared with the Ethanol yield with enzymes from Y and Z. Thus results shows that enzyme from X is best suited cellulase complex for cellulose conversion thus producing significantly higher amount of ethanol when work along with thermotolerantS. Cerevisiae with simultaneous saccharification and fermention process when substrate is wheat straw.
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Thesis M.Tech (R) National Institute of Technology, Rourkela

Cellulases and related hydrolytic enzymes represent a key cost factor for biochemical conversion of cellulosic biomass feedstocks to sugars for biofuels and chemical production. The performance of benchmark cellulose preparations produced by enzymes to convert pretreated wheat straw cellulose to glucose and fermentation to produce ethanol was evaluated under common experimental conditons and is reported here in a non-attributed manner.
In the study, inspections were carried out with three commercial cellulose complexes produced by X, Y and Z enzymes. These set of enzymes were tested on pretreated wheat straw for enzymatic hydrolysis and ethanol production with simultaneous saccharification and fermention. For enzymatic hydrolysis, pretreated wheat straw (loaded at10% w/w ) was hydrolyzed with all three complexes at varied enzyme concentration ( 1 mg -30 mg protein/g of biomass, 50◦C, pH 5) separately in 250 ml roller bottles. Same set of enzymes were also used along with thermotolerantyeast (Saccharomycescerevisiae2034 ; 38◦C, pH 5) to produce ethanol using simultaneous saccharification and fermention process in 250 ml shak flask.
In enzymatic hydrolysis, enzyme from X was the best performer ( high glucose yield at lower enzyme (protein) loadings) with up to95% glucose yield at roughly 10-15 mg protein/g cellulose , when compared with the enzymes from Y and Z. In simultaneous saccharification and fermention, Ethanol yield with enzyme from X was upto 80% at 20 mg protein/g cellulose which was comparably high when compared with the Ethanol yield with enzymes from Y and Z.
Thus results shows that enzyme from X is best suited cellulase complex for cellulose conversion thus producing significantly higher amount of ethanol when work along with thermotolerantS. Cerevisiae with simultaneous saccharification and fermention process when substrate is wheat straw.

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