Human stem cell manual [electronic resource] : a laboratory guide / edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz.

Contributor(s): Loring, Jeanne Frances, 1950- | Wesselschmidt, Robin L | Schwartz, Philip HMaterial type: TextTextPublisher: Amsterdam ; New York : Academic Press, 2007Description: 1 online resource (xxiii, 461 p.) : ill. (some col.)ISBN: 9780123704658; 0123704650; 9780080549880 (electronic bk.); 0080549888 (electronic bk.)Subject(s): Stem cells -- Laboratory manuals | Embryonic stem cells | Biology -- Research -- Methodology | Stem Cells -- Laboratory Manuals | Embryonic Stem Cells | Adult Stem Cells | Biomedical Research -- methods | MEDICAL -- ResearchGenre/Form: Electronic books.Additional physical formats: Print version:: Human stem cell manual.DDC classification: 616/.02774 LOC classification: QH588.S83 | H86 2007Online resources: ScienceDirect
Contents:
Foreword by James Battey -- Foreword by Jeannie Fontana -- Preface -- Part I: Basic methods: Maintenance of stem cell cultures -- 1. Human Embryonic Stem Cell Culture -- 2. Feeder layers and feeder-free culture -- 3. Mouse Embryonic Fibroblast Feeder Cells -- 4. Cryopreservation of hESC -- Part II: Basic methods: Characterization of stem cells -- 5. Classical Cytogenetics: Karyotyping -- 6. Spectral Karyotyping and Fluorescent in situ Hybridization -- 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns -- 8. FACS/Cell sorting -- 9. Fluorescence Immunocytochemical Analysis of Stem Cells -- 10. Characterization of Stem Cells Using RT-PCR -- 11. Gene Expression Profiling of Stem Cells by Microarray -- 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice -- 13. Generation of HESC-Derived Teratomas -- Part III: Differentiation of stem cells -- 14. Embryoid Body Formation and Neuroepithelia Differentiation -- 15. Motor Neuron and Dopamine Neuron Differentiation -- 16. Oligodendrocyte Differentiation from hESC -- 17. Cardiac -- 18. Hematopoietic -- Part IV: Genetic manipulation of stem cells -- 19. Genetic Manipulation of hESC: Lentivirus Vectors -- 20. Methods developed for mouse ES cells -- Part V: Advanced methods -- 21. Derivation of human ES cells -- 22. Neural Stem Cell Culture -- 23. Transplantation of stem cells -- 24. In vitro fertilization -- 25. Functional characterization-neural cells -- Part VI: Practical issues in establishing a human ES cell laboratory -- 26. Setting Up a Facility for hESC Research -- Part VII: Essays on stem cell biology -- 27. Intellectual Property: Owning the Stem Cell -- 28. Ethical Concerns for Stem Cell Research -- 29. Guidelines for hESC Research Oversight (ESCRO) Committees.
Basic methods in stem cell culture -- Characterization of stem cells -- Differentiation of human embryonic cells -- Genetic manipulation of stem cells -- Advanced methods -- Setting up a laboratory -- Stem cells and society.
Summary: Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.
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Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.

Foreword by James Battey -- Foreword by Jeannie Fontana -- Preface -- Part I: Basic methods: Maintenance of stem cell cultures -- 1. Human Embryonic Stem Cell Culture -- 2. Feeder layers and feeder-free culture -- 3. Mouse Embryonic Fibroblast Feeder Cells -- 4. Cryopreservation of hESC -- Part II: Basic methods: Characterization of stem cells -- 5. Classical Cytogenetics: Karyotyping -- 6. Spectral Karyotyping and Fluorescent in situ Hybridization -- 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns -- 8. FACS/Cell sorting -- 9. Fluorescence Immunocytochemical Analysis of Stem Cells -- 10. Characterization of Stem Cells Using RT-PCR -- 11. Gene Expression Profiling of Stem Cells by Microarray -- 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice -- 13. Generation of HESC-Derived Teratomas -- Part III: Differentiation of stem cells -- 14. Embryoid Body Formation and Neuroepithelia Differentiation -- 15. Motor Neuron and Dopamine Neuron Differentiation -- 16. Oligodendrocyte Differentiation from hESC -- 17. Cardiac -- 18. Hematopoietic -- Part IV: Genetic manipulation of stem cells -- 19. Genetic Manipulation of hESC: Lentivirus Vectors -- 20. Methods developed for mouse ES cells -- Part V: Advanced methods -- 21. Derivation of human ES cells -- 22. Neural Stem Cell Culture -- 23. Transplantation of stem cells -- 24. In vitro fertilization -- 25. Functional characterization-neural cells -- Part VI: Practical issues in establishing a human ES cell laboratory -- 26. Setting Up a Facility for hESC Research -- Part VII: Essays on stem cell biology -- 27. Intellectual Property: Owning the Stem Cell -- 28. Ethical Concerns for Stem Cell Research -- 29. Guidelines for hESC Research Oversight (ESCRO) Committees.

Includes bibliographical references and index.

Basic methods in stem cell culture -- Characterization of stem cells -- Differentiation of human embryonic cells -- Genetic manipulation of stem cells -- Advanced methods -- Setting up a laboratory -- Stem cells and society.

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