Molecular biology [electronic resource] : a project approach / Susan J. Karcher.

By: Karcher, Susan JMaterial type: TextTextPublisher: San Diego : Academic Press, c1995Description: 1 online resource (xxi, 280 p.) : illISBN: 9780123977205; 0123977207; 9780080536835 (electronic bk.); 0080536832 (electronic bk.)Subject(s): Molecular biology -- Laboratory manuals | Molecular biology -- Experiments | Molecular Biology -- Laboratory Manuals | Biologie mol�eculaire -- Manuels de laboratoire | Biologie mol�eculaire -- Exp�eriences | SCIENCE -- Life Sciences -- Molecular Biology | Molecular biology -- Laboratory manuals | Molecular biologyGenre/Form: Electronic books.Additional physical formats: Print version:: Molecular biology.DDC classification: 574.8/8/078 LOC classification: QH506 | .K367 1995ebOnline resources: ScienceDirect
Contents:
Transposon Mutagenesis of Escherichia coli. -- Recombinant DNA and Cloning. -- Southern Blot Analysis. -- Plant Genomic Southern Blotting with Probes for Low- and High-Copy-Number Genes. -- RNA Purification and Northern Blot Analysis. -- Polymerase Chain Reaction. -- Appendices. -- Glossary. -- Subject Index.
Summary: This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Key Features * Includes a glossary of over 200 terms important for understanding molecular biology * Uses an inexpensive source of eukaryotic cells - great for schools on a budget * Includes Methods Locator that provides instant access to the latest methods * Contain clearly written, easy-to-follow, student-tested instructions: * Sterile techniques * Phage titration * Gel electrophoresis of DNA * Restriction enzyme digestion * Plasmid isolation * Transformation of E. Coli * Recombinant DNA cloning * Nick translation labeling * Nonradioactive primer labelling * Nonradioactive DNA detection * Southern blotting * Colony hybridization * Purification of plant DNA * RNA purification * Northern blotting * Purification of poly A+ RNA * Polymerase chain reaction (PCR).
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This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Key Features * Includes a glossary of over 200 terms important for understanding molecular biology * Uses an inexpensive source of eukaryotic cells - great for schools on a budget * Includes Methods Locator that provides instant access to the latest methods * Contain clearly written, easy-to-follow, student-tested instructions: * Sterile techniques * Phage titration * Gel electrophoresis of DNA * Restriction enzyme digestion * Plasmid isolation * Transformation of E. Coli * Recombinant DNA cloning * Nick translation labeling * Nonradioactive primer labelling * Nonradioactive DNA detection * Southern blotting * Colony hybridization * Purification of plant DNA * RNA purification * Northern blotting * Purification of poly A+ RNA * Polymerase chain reaction (PCR).

Transposon Mutagenesis of Escherichia coli. -- Recombinant DNA and Cloning. -- Southern Blot Analysis. -- Plant Genomic Southern Blotting with Probes for Low- and High-Copy-Number Genes. -- RNA Purification and Northern Blot Analysis. -- Polymerase Chain Reaction. -- Appendices. -- Glossary. -- Subject Index.

Includes bibliographical references and index.

Description based on print version record.

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