Techniques in protein chemistry VI [electronic resource] / edited by John W. Crabb.Material type: TextPublisher: San Diego : Academic Press, c1995Description: 1 online resource (xix, 585 p.) : illISBN: 9780121947125; 0121947122; 9780080542263 (electronic bk.); 0080542263 (electronic bk.)Other title: Techniques in protein chemistry 6Subject(s): Proteins -- Analysis -- Congresses | Peptides -- Analysis -- Congresses | Nucleotide sequence -- Congresses | Amino Acid Sequence -- Congresses | Molecular Structure -- Congresses | Proteins -- analysis -- Congresses | SCIENCE -- Life Sciences -- Biochemistry | Proteins Chemical propertiesGenre/Form: Electronic books.Additional physical formats: Print version:: Techniques in protein chemistry VI.DDC classification: 574.19/245 LOC classification: QP551 | .T338 1995ebOnline resources: ScienceDirect
TECHNIQUES IN PROTEIN CHEMISTRY VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: Protein sequencing and amino acid analysis Mass spectral analysis of peptides and proteins Posttranslational processing High-sensitivity protein and peptide separations Protein folding and NMR Analysis of protein interactions Protein design and engineering Techniques in Protein Chemistry VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: * Protein sequencing and amino acid analysis * Mass spectral analysis of peptides and proteins * Posttranslational processing * High-sensitivity protein and peptide separations * Protein folding and NMR * Analysis of protein interactions * Protein design and engineering.
Foreword. -- Preface. -- Acknowledgements. -- Mass Spectrometry of Peptides and Proteins: -- The Use of a Volatile N-Terminal Degradation Reagent for Rapid, High-Sensitivity Sequence Analysis of Peptides by Generation of Sequences Ladders, M. Bartlet-Jones, W.A. Jeffery, H.F. Hansen, and D.J.C. Pappin. -- Investigation of Polyethylene Membranes as Potential Sample Supports for Linking SDS-PAGE with MALDI-TOF MS for the Mass Measurement of Proteins, J.A. Blackledge and A.J. Alexander. -- Comparison of ESI-MS, LSIMS, and MALDI-TOF-MS for the Primary Structure Analysis of a Monoclonal Antibody, L. Cano, K.M. Swiderek, and J.E. Shively. -- MS Based Scanning Methodologies Applied to Conus Venom, A.G. Craig, W.H. Fischer, J.E. Rivier, J.M. McIntosh, and W.R. Gray. -- Direct Coupling of an Automated 2-Dimensional Microcolumn Affinity Chromatography-Capillary HPLC System with Mass Spectrometry for Biomolecule Analysis, D.B Kassell, T.G. Consler, M. Shalaby, P. Sekhri, N. Gordon, and T. Nadler. -- Edman Degradation and MALDI Sequencing Enables N- and C-Terminal Sequence Analysis of Peptides, R. Kellner, G. Talbo, T. Houthaeve, and M. Mann. -- Identification of the Amino Terminal Peptide of N-Terminally Blocked Proteins by Differential Deutero-Acetylation Using LC/MS Techniques, C.D. Thulin and K.A. Walsh. -- Analysis of Posttranslational Processing Events: -- HPAEC-PAD Analysis of Monoclonal Antibody Glycosylation, J. Rohrer, J. Thayer, N. Avdalovic, and M. Weitzhandler. -- Carbohydrate Structure Characterization of Two Soluble Forms of a Ligand for the ECK Receptor Tyrosine Kinase, C.L. Clogston, P.L. Derby, R. Toso, J.D. Skrine, M. Zhang, V. Parker, G.M. Fox, T.D. Bartley, and H.S. Lu. -- Characterization of Individual N- and O-Linked Glycosylation Sites Using Edman DegradationA. A. Gooley, N.H. Packer, A. Pisano, J.W. Redmond, K.L. Williams, A. Jones, M. Loughnan, and P.F. Alewood. -- The Unexpected Presence of Hydroxylysine in Noncollagenous Proteins, M.S. Molony, S.-L. Wu, L.K. Keyt, and R.J. Harris. -- Isolation of Escherichia coli Synthesized Recombinant Proteins that Contain (e>-N-Acetyllysine, B.N. Violand, M.R. Schlittler, C.Q. Lawson, J.F. Kane, N.R. Siegel, C.E. Smith, and K.L. Duffin. -- LC-MS Methods for Selective Detection of Posttranslational Modifications in Proteins: Glycosylation, Phosphorylation, Sulfation, and Acylation, M.F Bean, R.S. Annan, M.E. Hemling, M. Mentzer, M.J. Huddleston, and S.A. Carr. -- Identification of Phosphorylation Sites by Edman DegradationJ. D. Shannon and J.W. Fox. -- Determination of the Disulfide Bonds of Human Macrophage Chemoattractant Protein-1 Using a Gas Phase Sequencer, R. Seetharam, J.I. Corman, and S.M. Kamerkar. -- Protein Sequencing and Amino Acid Analysis: -- Enzymatic Digestion of PVDF-Bound Proteins in the Presence of Glucopyranoside Detergents: Applicability to Mass Spectrometry, J. Fernandez, F. Gharahdaghi, and S.M. Mische. -- In-Gel Digestion of SDS PAGE-Separated Proteins: Observations from Internal Sequencing of 25 Proteins, K.R. Williams and K.L. Stone. -- Peptide Mapping at the 1 m> Level: In-Gel vs. PVDF Digestion Techniques, L.A. Merewether, C.L. Clogston, S.D. Patterson, and H.S. Lu. -- Enzymatic Digestion of Proteins in Zinc Chloride and Ponceau S Stained Gels, S. Zhou and A. Admon. -- Direct Collection Onto Zitex and PVDF for Edman Sequencing: Elimination of Polybrene, W.A. Burkhart, M.B. Moyer, W.M. Bodnar, A.M. Everson, V.G. Valladares, and J.M. Bailey. -- Minimizing N-to-O Shift in Edman Sequencing, W.H. Vensel and G.E. Tarr. -- The Hydrolysis Process and the Quality of Amino Acid Analysis: ABRF-94AAA Collaborative Trial, K. �U, Y�uksel, T.T. Andersen, I. Apostol, J.W. Fox, R.J. Paxton, and D.J. Strydom. -- A New Reagent for Cleaving at Cystine Residues, C. Mitchell, L. Hinman, L. Miller, and P.C. Andrews. -- Protein Sequence Analysis Using Microbore PTH Separations, M.F. Rohde, C. Clogston, L.A. Merewether, P. Derby, and K.D. Nugent. -- Assignment of Cysteine and Tryptophan Residues during Protein Sequencing: Results of ABRF-94 SEQ, J. Gambee, P.C. Andrews, K. DeJongh, G. Grant, B. Merrill, S. Mische, and J. Rush. -- Automated C-Terminal Protein Sequence Analysis Using the Hewlett-Packard G1009A C-Terminal Protein Sequencing System, C.G. Miller, D.H. Hawke, J. Tso, and S. Early. -- Applications Using an Alkylation Method for Carboxy-Terminal Protein Sequencing, M.L. Bozzini, J. Zhao, P.-M. Yuan, D. Ciolek, Y.-C. Pan, J. Horton, D.R. Marshak, and V.L. Boyd. -- C-Terminal Sequence Analysis of Polypeptides Containing C-Terminal Proline, J.M. Bailey, O. Tu, G. Issai, and J.E. Shively. -- Peptide and Protein Separations and Other Methods: -- High Sensitivity Detection of Tryptic Digests Using Derivatization and Fluorescence Detection, S.A. Cohen, I. Mechnikov, and P. Young. -- Reagents for Rapid Reduction of Disulfide Bonds in Proteins, R. Singh and G.M. Whitesides. -- Strategies for the Removal of Ionic and Nonionic Detergents from Protein and Peptide Mixtures for On- and Off-Line Liquid Chromatography Mass Spectrometry (LCMS), K.M. Swiderek, M.L. Klein, S.A. Hefta, and J.E. Shively. -- Online Preparation of Complex Biological Samples prior to Analysis by HPLC, LC/MS, and /or Protein Sequencing, K. Stoney and K. Nugent. -- Methods for the Purification and Characterization of Calcium-Binding Proteins from Retina, A.S. Polans, K. Palczewski, W.A. Gorczyca, and J.W. Crabb. -- Evidence for the Presence of a>-Bungarotoxin in Venom-Derived k>-Bungarotoxin, J.J. Fiordalisi and G.A. Grant. -- Progress in the Development of Solvent and Chromatography Systems Appropriate for Bitopic Membrane Proteins, S.-J. Kil, L.M. Oleksa, G.C. Landis, and C.R. Sanders, II.
Rapid Separation of Proteins and Peptides Using Conventional Silica-Based Supports: Identification of 2-D Gel Proteins following In-Gel Proteolysis, R.L. Moritz, J. Eddes, H. Ji, G.E. Reid, and R.J. Simpson. -- Mutagenesis and Protein Design: -- Studying a>-Helix and b>-Sheet Formation in Small Proteins, C.K. Smith, M. Munson, and L. Regan. -- Circular Permutation of RNase T1 through PCR Based Site-Directed Mutagenesis, J.M. Kuo, L.S. Mullins, J.B. Garrett, and F.M. Raushel. -- E. coli-Expressed Human Neurotrophin-3 Characterization of a C-Terminal Extended Product, J.O. Hui, S.-Y. Meng, V. Katta, L. Tsai, M.F. Rohde, and M. Haniu. -- Spectral Enhancement of Recombinant Proteins with Tryptophan Analogs: The Soluble Domain of Human Tissue Factor, C.A. Hasselbacher, R. Rusinova, E. Rusinova, and J.B.A. Ross. -- Utilization of Partial Reactions, Side Reactions, and Chemical Rescue to Analyze Site-Directed Mutants of Ribulose 1,5-Bisphosphate (RuBP) Carboxylase/Oxygenase (Rubisco), M.R. Harpel, E.H. Serpersu, and F.C. Hartman. -- Probing the Roles of Conserved Histidine Residues in b>-Galactosidase (E. coli) Using Site Directed Mutagenesis and Transition State Analog Inhibition, N.J. Roth, K.Y.N. Wong, and R.E. Huber. -- Analysis of Protein Interactions: -- Rapid in Vitro Assembly of Class I Major Histocompatibility Complex, N.J. Papadopoulos, J.C. Sacchettini, S.G. Nathenson, and R.H. Angeletti. -- Peptide Models of bZIP Proteins: Quantitative Analysis of DNA Affinity and Specificity, S.J. Metallo and A. Schepartz. -- Applying Affinity Coelectrophoresis to the Study of Nonspecific, DNA Binding Peptides, M.L. Nedved and G.R. Moe. -- Investigating Calmodulin-Target Sequence Interactions Using Mutant Proteins and Synthetic Target Peptides, W.A. Findlay, S.R. Martin, and P.M. Bayley. -- Interactions of Bacterial Cell-Surface Proteins with Antibodies: A Versatile Set of Protein-Protein Interactions, G.C.K. Roberts, L.-Y. Lian, I.L. Barsukov, J.P. Derrick, K. Kato, and Y. Arata. -- Studies of Cytokine-Cytokine Receptor Interactions: Influence of Ligand Dimerization, L.D. Ward, G.J. Howlett, R.L. Moritz, A. Hammacher, K. Yasukawa, and R.J. Simpson. -- New High Sensitivity Sedimentation Methods: Application to the Analysis of the Assembly of Bacteriophage P22, W.F. Stafford, III, S. Liu, and P.E. Prevelige, Jr. -- Protein Conformation and Folding: -- Cyanogen as a Conformational Probe, R.A. Day, A. Hignite, and W.E. Gooden. -- Evaluation of Interactions between Residues in a a>-Helices by Exhaustive Conformational Search, T.P. Creamer, R. Srinivasan, and G.D. Rose. -- Design, Synthesis, and Characterization of a Water-Soluble b>-Sheet Peptide, D.S. Wishart, L.H. Kondejewski, P.D. Semchuk, C.M. Kay, R.S. Hodges, and B.D. Sykes. -- Automated Analysis of Protein Folding, R.A. Smith, J. Henkin, and T.F. Holzman. -- HSP70 Protein Complexes: Their Characterization by Size-Exclusion HPLC, D.R. Palleros, L. Shi, K. Reid, and A. Fink. -- Methods for Collecting and Analyzing Attenuated Total Reflectance FTIR Spectra of Proteins in Solution, K.A. Oberg and A.L. Fink. -- NMR Analysis of Peptides and Proteins: -- <sup>19</sup>F NMR Studies of Fluorinated Sugars Binding to the Glucose and Galactose Receptor, L.A. Luck. -- Heteronuclear Gradient-Enhanced NMR for the Study of 20-30 kDa Proteins: Application to Human Carbonic Anhydrase II, R.A. Venters and L.D. Spicer. -- Toward the Solution Structure of Large (>30 kDa) Proteins and Macromolecular Complexes, C.H. Arrowsmith, W. Lee, M. Revington, T. Yamazaki, and L.E. Kay. -- Solution Structures of Horse Ferro- and Ferricytochrome c Using 2D and 3D <sup>1</sup>H NMR and Restrained Simulated Annealing, P.X. Qi, E.J. Fuentes, R.A. Beckman, D.L. Di Stefano, and A.J. Wand. -- NMR Relaxation Methods to Study Ligand-Receptor Interactions, D.W. Hoyt, J.-J. Wang, and B.D. Sykes. -- Peptide Synthesis: -- Application of 2-Chlorotrityl Resin: Simultaneous Synthesis of Peptides Which Differ in the C-Termini, A.L. Hong, T.T. Le, and T. Phan. -- Correlation of Cleavage Techniques with Side-Reactions following Solid-Phase Peptide Synthesis, G.B. Fields, R.H. Angeletti, L.F. Bonewald, W.T. Moore, A.J. Smith, J.T. Stults, and L.C. Williams. -- Protein Synthesis on a Solid Support Using Fragment Condensation, S. Brandtner and C. Griesinger. -- Characterization of a Side Reaction Using Stepwise Detection in Peptide Synthesis with Fmoc Chemistry, Y. Yang, W.V. Sweeney, S. Th�ornqvist, K. Schneider, B.T. Chait, and J.P. Tam. -- Erratum: -- High Sensitivity Peptide Sequence Analysis Using in Situ Proteolysis on High Retention PVDF Membranes and a Biphasic Reaction Column Sequencer, S. Best, D.F. Reim, J. Mozdzanowski, and D.W. Speicher. -- Index.
"Published under the auspices of the Protein Society"--Cover.
Most of the papers were obtained from poster sessions of the Eighth Symposium of the Protein Society, held in San Diego July 9-13, 1994.
Includes bibliographical references and index.
Description based on print version record.