RNA methodologies [electronic resource] : a laboratory guide for isolation and characterization / Robert E. Farrell, Jr.

By: Farrell, Robert EMaterial type: TextTextPublisher: Amsterdam ; Boston : Elsevier/Academic Press, c2005Edition: 3rd edDescription: 1 online resource (xxv, 767 p.) : illISBN: 9780122496967; 0122496965; 0080454763 (electronic bk.); 9780080454764 (electronic bk.)Subject(s): RNA -- Analysis -- Laboratory manuals | RNA -- isolation & purification -- Laboratory Manuals | Molecular Biology -- methods -- Laboratory Manuals | RNA -- physiology -- Laboratory Manuals | ARN -- Analyse -- Manuels de laboratoire | SCIENCE -- Life Sciences -- Molecular Biology | RNA | LaboratoriumonderzoekGenre/Form: Electronic books.Additional physical formats: Print version:: RNA methodologies.DDC classification: 572.8/8 LOC classification: QP623 | .F37 2005ebOnline resources: ScienceDirect
Contents:
RNA and the cellular biochemistry revisited -- Transcription and the organization of eukaryotic genes -- Messenger RNA -- Resilient ribonucleases -- RNA isolation strategies -- The truth about tissues -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Dot blot analysis -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantification of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR -- Quantitative PCR techniques -- Transcript subtraction methods -- mRNA differential display -- High-throughput analysis of gene expression -- RNA interference : targeted gene silencing -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA Paradigm -- Epilogue : a few pearls of wisdom.
Summary: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5 and 3 RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects.
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Includes bibliographical references and index.

RNA and the cellular biochemistry revisited -- Transcription and the organization of eukaryotic genes -- Messenger RNA -- Resilient ribonucleases -- RNA isolation strategies -- The truth about tissues -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Dot blot analysis -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantification of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR -- Quantitative PCR techniques -- Transcript subtraction methods -- mRNA differential display -- High-throughput analysis of gene expression -- RNA interference : targeted gene silencing -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA Paradigm -- Epilogue : a few pearls of wisdom.

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5 and 3 RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects.

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