Phage display of peptides and proteins [electronic resource] : a laboratory manual / edited by Brian K. Kay, Jill Winter, John McCafferty.

Contributor(s): Kay, Brian K | Winter, Jill | McCafferty, John, DrMaterial type: TextTextPublisher: San Diego : Academic Press, 1996Description: 1 online resource (xxii, 344 p.) : ill. (some col.)ISBN: 9780124023802; 0124023800; 9780080538709 (electronic bk.); 0080538703 (electronic bk.)Subject(s): Bacteriophages -- Laboratory manuals | Microbial biotechnology -- Laboratory manuals | Viral proteins -- Laboratory manuals | Affinity chromatography -- Laboratory manuals | Peptides -- Biotechnology -- Laboratory manuals | Proteins -- Biotechnology -- Laboratory manuals | Bacteriophages -- Laboratory Manuals | Peptides -- Laboratory Manuals | Proteins -- Laboratory Manuals | Chromatography, Affinity -- Laboratory Manuals | SCIENCE -- Life Sciences -- Evolution | Genexpressie | Combinatorische chemie | Peptiden | Eiwitten | Antistoffen | Bacteriophages -- Laboratory manuals | Microbial biotechnology -- Laboratory manuals | Viral proteins -- Laboratory manuals | Affinity chromatography -- Laboratory manuals | Peptides -- Biotechnology -- Laboratory manuals | Proteins -- Biotechnology -- Laboratory manuals | Microbiology Laboratory techniquesGenre/Form: Electronic books.Additional physical formats: Print version:: Phage display of peptides and proteins.DDC classification: 576/.6482 LOC classification: QR342 | .P456 1996ebOther classification: 42.15 | 35.73 Online resources: ScienceDirect
Contents:
Foreword. Biology of the Filamentous Bacteriophage. Principles and Applications of Phage Display. Vectros for Phage Display. Construction of Random Peptide Libraries in Bacteriophage. Construction and Screening of Antibody Display Libraries. Phagemeid-Displayed Peptide Libraries. Phage Libraries Displaying Random Peptides. Derived Form a Target Sequence. Display and Selection of Proteins on Genetic Packages. A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand Interactions Made Possible By Linkage of Recogntion and Replication Functions. Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design. Use of Filamentous Virus Particles as Immunogens. Screening Phage Displayed Random Peptide Libraries. Phage Display Factors Affecting Planning Efficieny. Preparation of Second Generation Phage Libraries. Nonradioactive Sequencing of Random Peptide Recombinant Phage. Measurement of Peptide Binding Affinities Using Fluorescence Polarization.
Summary: Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Features * Prepared by experts in the field * Provides proven laboratory protocols, troubleshooting, and tips * Includes maps, sequences, and sample data * Contains extensive and up-to-date references.
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Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Features * Prepared by experts in the field * Provides proven laboratory protocols, troubleshooting, and tips * Includes maps, sequences, and sample data * Contains extensive and up-to-date references.

Foreword. Biology of the Filamentous Bacteriophage. Principles and Applications of Phage Display. Vectros for Phage Display. Construction of Random Peptide Libraries in Bacteriophage. Construction and Screening of Antibody Display Libraries. Phagemeid-Displayed Peptide Libraries. Phage Libraries Displaying Random Peptides. Derived Form a Target Sequence. Display and Selection of Proteins on Genetic Packages. A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand Interactions Made Possible By Linkage of Recogntion and Replication Functions. Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design. Use of Filamentous Virus Particles as Immunogens. Screening Phage Displayed Random Peptide Libraries. Phage Display Factors Affecting Planning Efficieny. Preparation of Second Generation Phage Libraries. Nonradioactive Sequencing of Random Peptide Recombinant Phage. Measurement of Peptide Binding Affinities Using Fluorescence Polarization.

Includes bibliographical references and index.

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