MRNA formation and function [electronic resource] / edited by Joel D. Richter.

Contributor(s): Richter, Joel DMaterial type: TextTextPublisher: San Diego : Academic Press, c1997Description: 1 online resource (xvii, 395 p., [2] p. of plates) : ill. (some col.)ISBN: 9780125875455; 0125875452; 9780080537047 (electronic bk.); 0080537049 (electronic bk.); 1281119326; 9781281119322Subject(s): Messenger RNA -- Research -- Methodology | RNA, Messenger | Research -- methods | ARN messager | SCIENCE -- Life Sciences -- Molecular Biology | ARN messagers | RNA, Messenger -- Research -- MethodologyGenre/Form: Electronic books.Additional physical formats: Print version:: MRNA formation and function.DDC classification: 572.8/8 LOC classification: QP623.5.M47 | M75 1997ebOnline resources: ScienceDirect
Contents:
C. Sune, P.R. Bohjanen, Y. Liu, M.A. Garcia-Blanco, and S.F. Jamison, The Tat-TAR RNP, a Master Switch That Regulates HIV-1 Gene Expression. -- C.R. Miller, S.F. Jamison, and M.A. Garcia-Blanco, HeLa Nuclear Extract: Amodified Protocol. -- J. Valcarcel, C. Martinez, and M.R. Green, Functional Analysis of Splicing Factors and Regulators. -- J.G. Patton, B.T. Dye, D.C. Barnard, and J.G. McAfee, Identification of pre-mRNA Splicing Factors and Analysis of RNA-Protein Interaction. -- G. Gilmartin, In Vitro Analysis of Mammalian Cell mRNA 3 Processing. -- J. Wilusz, Rapid Identification and Cloning of Sequence-Specific RNA Binding Proteins. -- J.S. Butler, M.W. Briggs, and A. Proweller, Analysis of Polyadenylation Phenotypes in Saccharomyces cerevisiae. -- P.D. Gershon, Poly(A) Polymerase/cap-Specific 2-O-Methyltransferase from Vaccinia Virus: Expression, Purification, Uses, and Protein-Ligand Interaction Assays. -- A.N. Hennigan and A. Jacobson, A Genetic Approach to Mapping Coding Region Determinants of mRNA Instability in Yeast. -- W.F. Marzluff, M.L. Whitfield, Z. Dominski, and Z.-F. Wang, Identification of the Protein Which Interacts with the 3 End of Histone mRNA. -- M. Holcik and S.A. Liebhaber, Analysis of mRNP Complexes Assembled in Vitro. -- A. Laird-Offringa, Analysis of RNA-Binding Proteins Using in Vitro Genetics. -- L.G. Andrews and J.D. Keene, Interactions of Proteins with Specific Sequences in RNA. -- C. Jain and J.G. Belasco, A Rapid Genetic Method for the Study of RNA Binding Proteins. -- P. Ansel-McKinney and L. Gehrke, Footprinting RNA-Protein Complexes with Hydroxyl Radicals. -- H. Ruan, C.Y. Brown, and D.R. Morris, Analysis of Ribosome Loading onto mRNA Species: Implications for Translational Control. -- G. Belsham, Analysis of Picornavirus Internal Ribosome Entry Site Function in Vivo. -- M.R. Jacobson and T. Pederson, RNA Traffic and Localization Reproted by Fluorescent Molecular Cytochemistry in Living Cells. -- A. Manoukian, Detection of mRNA in Situ: Techniques for Studying Gene Expression in Drosophila melanogaster Tissues. -- M.H. Jacob and O.C. Ikonomov, Differential Display Protocol That Preferentially Identifies mRNAs of Moderate to Low Abundance in a Microscopic System. -- Subject Index.
The Tat-TAR RNP, a master switch that regulates HIV-1 gene expression--HeLa nuclear extract: a modified protocol--Functional analysis of splicing factors and regulators--Identification of pre-mRNA splicing factors and analysis of RNA-protein interaction--In Vitro analysis of mammalian cell mRNA 3' processing--Rapid identification and cloning of sequence-specific RNA binding proteins--Analysis of polyadenylation phenotypes in Saccharomyces cerevisiae--Poly(A) polymerase/Cap-specific 2'-O-methyltransferase from Vaccinia virus: expression, purification, uses, and protein-ligand interaction assays--A genetic approach to mapping coding region determinants of mRNA instability in yeast--Identification of the protein that interacts with the 3' end of histone mRNA--Analysis of mRNP complexes assembled in Vitro--Analysis of RNA binding proteins using in Vitro genetics--Interactions of proteins with specific sequences in RNA--A rapid genetic method for the study of RNA binding proteins--Footprinting RNA-protein complexes with hydroxyl radicals--Analysis of ribosome loading onto mRNA species: implications for translational control--Analysis of Picornavirus internal ribosome entry site function in Vivo--RNA traffic and localization reported by fluorescent molecular cytochemistry in living cells--
Detection of mRNA in Situ: techniques for studying gene expression in Drosophila melanogaster tissues--Differential display protocol that preferentially identifies mRNAs of moderate to low abundance in a microscopic system.
Summary: MRNA Formation and Function presents a compendium of techniques geared exclusively toward the understanding of RNA metabolism. It will be particularly useful because a number of different organisms and systems are employed. Key Features * Isolation and characterization of specific RNA binding proteins * RNA metabolism and associated regulatory proteins * RNA detection and localization * A genetic approach to RNA function.
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MRNA Formation and Function presents a compendium of techniques geared exclusively toward the understanding of RNA metabolism. It will be particularly useful because a number of different organisms and systems are employed. Key Features * Isolation and characterization of specific RNA binding proteins * RNA metabolism and associated regulatory proteins * RNA detection and localization * A genetic approach to RNA function.

C. Sune, P.R. Bohjanen, Y. Liu, M.A. Garcia-Blanco, and S.F. Jamison, The Tat-TAR RNP, a Master Switch That Regulates HIV-1 Gene Expression. -- C.R. Miller, S.F. Jamison, and M.A. Garcia-Blanco, HeLa Nuclear Extract: Amodified Protocol. -- J. Valcarcel, C. Martinez, and M.R. Green, Functional Analysis of Splicing Factors and Regulators. -- J.G. Patton, B.T. Dye, D.C. Barnard, and J.G. McAfee, Identification of pre-mRNA Splicing Factors and Analysis of RNA-Protein Interaction. -- G. Gilmartin, In Vitro Analysis of Mammalian Cell mRNA 3 Processing. -- J. Wilusz, Rapid Identification and Cloning of Sequence-Specific RNA Binding Proteins. -- J.S. Butler, M.W. Briggs, and A. Proweller, Analysis of Polyadenylation Phenotypes in Saccharomyces cerevisiae. -- P.D. Gershon, Poly(A) Polymerase/cap-Specific 2-O-Methyltransferase from Vaccinia Virus: Expression, Purification, Uses, and Protein-Ligand Interaction Assays. -- A.N. Hennigan and A. Jacobson, A Genetic Approach to Mapping Coding Region Determinants of mRNA Instability in Yeast. -- W.F. Marzluff, M.L. Whitfield, Z. Dominski, and Z.-F. Wang, Identification of the Protein Which Interacts with the 3 End of Histone mRNA. -- M. Holcik and S.A. Liebhaber, Analysis of mRNP Complexes Assembled in Vitro. -- A. Laird-Offringa, Analysis of RNA-Binding Proteins Using in Vitro Genetics. -- L.G. Andrews and J.D. Keene, Interactions of Proteins with Specific Sequences in RNA. -- C. Jain and J.G. Belasco, A Rapid Genetic Method for the Study of RNA Binding Proteins. -- P. Ansel-McKinney and L. Gehrke, Footprinting RNA-Protein Complexes with Hydroxyl Radicals. -- H. Ruan, C.Y. Brown, and D.R. Morris, Analysis of Ribosome Loading onto mRNA Species: Implications for Translational Control. -- G. Belsham, Analysis of Picornavirus Internal Ribosome Entry Site Function in Vivo. -- M.R. Jacobson and T. Pederson, RNA Traffic and Localization Reproted by Fluorescent Molecular Cytochemistry in Living Cells. -- A. Manoukian, Detection of mRNA in Situ: Techniques for Studying Gene Expression in Drosophila melanogaster Tissues. -- M.H. Jacob and O.C. Ikonomov, Differential Display Protocol That Preferentially Identifies mRNAs of Moderate to Low Abundance in a Microscopic System. -- Subject Index.

Includes bibliographical references and index.

The Tat-TAR RNP, a master switch that regulates HIV-1 gene expression--HeLa nuclear extract: a modified protocol--Functional analysis of splicing factors and regulators--Identification of pre-mRNA splicing factors and analysis of RNA-protein interaction--In Vitro analysis of mammalian cell mRNA 3' processing--Rapid identification and cloning of sequence-specific RNA binding proteins--Analysis of polyadenylation phenotypes in Saccharomyces cerevisiae--Poly(A) polymerase/Cap-specific 2'-O-methyltransferase from Vaccinia virus: expression, purification, uses, and protein-ligand interaction assays--A genetic approach to mapping coding region determinants of mRNA instability in yeast--Identification of the protein that interacts with the 3' end of histone mRNA--Analysis of mRNP complexes assembled in Vitro--Analysis of RNA binding proteins using in Vitro genetics--Interactions of proteins with specific sequences in RNA--A rapid genetic method for the study of RNA binding proteins--Footprinting RNA-protein complexes with hydroxyl radicals--Analysis of ribosome loading onto mRNA species: implications for translational control--Analysis of Picornavirus internal ribosome entry site function in Vivo--RNA traffic and localization reported by fluorescent molecular cytochemistry in living cells--

Detection of mRNA in Situ: techniques for studying gene expression in Drosophila melanogaster tissues--Differential display protocol that preferentially identifies mRNAs of moderate to low abundance in a microscopic system.

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