Biomedical electron microscopy [electronic resource] : illustrated methods and interpretations / Arvid B. Maunsbach, Bj�orn A. Afzelius.

By: Maunsbach, Arvid BernhardContributor(s): Afzelius, Bj�ornMaterial type: TextTextPublisher: San Diego, Calif. : Academic Press, c1999Description: 1 online resource (xv, 548 p.) : ill. (some col.)ISBN: 9780124806108; 0124806104Subject(s): Electron microscopy -- Technique | Medical electronics | Cytodiagnosis -- methods -- Atlases | Microscopy, Electron -- methods -- Atlases | Electronics, Medical -- Atlases | Tissue Fixation -- methods -- AtlasesGenre/Form: Electronic books.Additional physical formats: Print version:: Biomedical electron microscopy.DDC classification: 570/.28/25 LOC classification: QH212.E4 | M38 1999ebOnline resources: ScienceDirect
Contents:
Preface. Acknowledgments. Micrograph Interpretation. Fixatives. Fixative Vehicle. Fixative Application. Dehydration and Embedding. Freezing and Freeze-Substitution. Support Films. Ultramicrotomy. Section-Staining. Microscopy. Image Recording. Photographic and Digital Printing. Negative Staining. Autoradiography. Cytochemistry. Immunocytochemistry. Freeze-Fracturing and Shadowing. Sampling and Quantitation. Image Processing. 3D Reconstructions. Appendix: Practical Methods. Index. Acknowledgment for Figures.
Summary: This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Key Features * Authored by the key leaders in the biological electron microscopy field * Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines * Introduces students on how to read and interpret electron micrographs.
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This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Key Features * Authored by the key leaders in the biological electron microscopy field * Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines * Introduces students on how to read and interpret electron micrographs.

Preface. Acknowledgments. Micrograph Interpretation. Fixatives. Fixative Vehicle. Fixative Application. Dehydration and Embedding. Freezing and Freeze-Substitution. Support Films. Ultramicrotomy. Section-Staining. Microscopy. Image Recording. Photographic and Digital Printing. Negative Staining. Autoradiography. Cytochemistry. Immunocytochemistry. Freeze-Fracturing and Shadowing. Sampling and Quantitation. Image Processing. 3D Reconstructions. Appendix: Practical Methods. Index. Acknowledgment for Figures.

Includes bibliographical references and index.

Description based on print version record.

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